Thursday, December 4, 2008

Bioanalyzer 2100

I had a chance to follow a KL team to attend a workshop in UNIMAS, basically they wanted to introduce a technology called Lab-on-Chip product, which is Bioanalyzer 2100 from Agilent.



This Bioanalyzer 2100 is a small machine, less than 10 Kg, is able to automate sizing and quantitation of DNA fragments, measure RNA quality, do protein analysis as well as cell counting. With this robust machine alone, a researcher can save cost and time, and spend more time on interpretation of results. It can replace the traditional agorose gel electrophoresis to quantitate the DNA fragment, do genotyping, need not to do SDS-PAGE, perform RFLP, pool samples for mutation detection and perform flow cytometry assays.

Any of these application, all you need is just to prepare your sample, and collect your data within 30 to 40 minutes. It's volatile, efficient, robust and user-friendly. This technology transform the traditional agarose gel into a gel matrix chip, which can accomodate about 12 samples. It needs only as low as 1 ul samples to get the analysis done. It even designed in such a way that to avoid cross-contamination between samples. *Different uses (DNA/RNA/Protein/Cell) needs different types of chips, labeled in different colors.

The exciting part will be on the uses of its software, it is designed in a way that depends on needs of users to interpret the same data differently. The technology is more sensitive that even we assume it's a smear band from an agorose gel, which can actually belongs to more tiny and close bands. This technology is thus more accurate.

The chips are come together with their reagents in a kit, which one kit has about 25 chips (1 chip for 12 samples) can used to run for 300 samples. Approximately, with 1 chip costs about RM12, you can calculate how much is per kit.

For more information, pls check here.

If any of you have heard or use this thing before, feel free to share with me. Enjoy these informations. =)


*I hope UNIMAS can buy one!! Fingers crossed.

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