Another month gone, and still, not much progress for my project, except that i going to run ELISA on august and want to extract RNA from all my stored blood samples and optimize the protocol. It is still a mess for me, especially i just sent over a few samples for bioanalyzer testing, and what returned is that very likely my RNAs were degraded. Even the highest concentration one the RIN only 4, and the rest either can't be detected any trace, or very low RIN. So, i would say it is start of my nightmare. I thought my sample preparation part is ok already, however, the low purity, low concentration and now the 'degradation' is distraught me right now. Ok, what should i do next, i have no idea.
About my personal life, still the same, except that i got one new pet at home. The puppy becomes so disobedient recently, every time i reached home, it will come to my feet and bite me! And when i open the door, it was so naughty and wanted to follow me into the house. I even closed the door almost flattened its nose, it still attempt to go in. Better think twice whether to keep it or not, i am very sure it will get more active when it grows up. Come back to my life after working hour, actually i am in "on call" status right now. All because i want to speed up my recruitment rate, so i told the nurses to call me when there is any putative patient coming in after working hours. In fact, this worked initially, as i got call when i was in my sweet dream and 11.45pm. This was a cool experience, pity to my parents because they got to accompany me as to protect me when this happened. However, my recruitment rate return to "zero" these two weeks, so my emotion is like roller coaster, high and low, excited and distressed. Not to say that actually i saw a patient went to coma and emergency condition, but a minute before he still consciously well; and my first time to see the hospital staff removed the dead body from the ward, with the wife stood restless watching her beloved.
Despite all these, i wonder how far i can go further. I am not doctor, not even a pharmacist or nurse. But if you said i am a researcher, because i am a postgrad in uni. I afraid i am not in that stage yet, i can't even decide when i should go uni, all the lab techniques and knowledge i have are blank in my head (because lack of practise). Being half of clinical, half of basic, i actually not belong to anywhere. Sad.
Ok, i know it is an irrational post. I will definitely look at a brighter side, maybe shall go marry first.




