Another month gone, and still, not much progress for my project, except that i going to run ELISA on august and want to extract RNA from all my stored blood samples and optimize the protocol. It is still a mess for me, especially i just sent over a few samples for bioanalyzer testing, and what returned is that very likely my RNAs were degraded. Even the highest concentration one the RIN only 4, and the rest either can't be detected any trace, or very low RIN. So, i would say it is start of my nightmare. I thought my sample preparation part is ok already, however, the low purity, low concentration and now the 'degradation' is distraught me right now. Ok, what should i do next, i have no idea.
About my personal life, still the same, except that i got one new pet at home. The puppy becomes so disobedient recently, every time i reached home, it will come to my feet and bite me! And when i open the door, it was so naughty and wanted to follow me into the house. I even closed the door almost flattened its nose, it still attempt to go in. Better think twice whether to keep it or not, i am very sure it will get more active when it grows up. Come back to my life after working hour, actually i am in "on call" status right now. All because i want to speed up my recruitment rate, so i told the nurses to call me when there is any putative patient coming in after working hours. In fact, this worked initially, as i got call when i was in my sweet dream and 11.45pm. This was a cool experience, pity to my parents because they got to accompany me as to protect me when this happened. However, my recruitment rate return to "zero" these two weeks, so my emotion is like roller coaster, high and low, excited and distressed. Not to say that actually i saw a patient went to coma and emergency condition, but a minute before he still consciously well; and my first time to see the hospital staff removed the dead body from the ward, with the wife stood restless watching her beloved.
Despite all these, i wonder how far i can go further. I am not doctor, not even a pharmacist or nurse. But if you said i am a researcher, because i am a postgrad in uni. I afraid i am not in that stage yet, i can't even decide when i should go uni, all the lab techniques and knowledge i have are blank in my head (because lack of practise). Being half of clinical, half of basic, i actually not belong to anywhere. Sad.
Ok, i know it is an irrational post. I will definitely look at a brighter side, maybe shall go marry first.
6 comments:
well, this is research, all the best.. :-p
mayb u should think of a way to do the isolation. i guess there's too little RNA extracted that's y the RIN value is low. Coz no 28S and 18S peaks observed in your chromatogram. And if it's RNA degradation, there should be a shift to the left, with peaks before 18s in the fast region
Don worry, u can learn from it. Never Give up and u will success~! Gambateh!
cklim: thanks, still think of what is the best way to solve out
lulu_ma: tri-reagent generally can give higher yield, if i switch to use the others, may be even worse. unless i pool the rna and reprecipitate it, any idea i can do it this way? thanks
Zh1ng: lol, learn while progressing and from mistakes, dun worry, i am fine. =)
hehe, when you will marry? tell us! XD
actually... we should not expect that what we learn from uni is showing the way we going to research life.
Education thought to us may be just a hard nutshell of what the real science is.
what important is the mindset we have in us.
a real scientist will have his/her extraordinary commitment into their life. regardless whether u got the educated background or not. It's just a matter of "I want it!" XD wakaka
paiseh, sorry for little marccus crapping XD
dear little marccus, i am fortunate to have your long comment in my blog post.. ^^, it is a very good comment.
In fact, i just came across a documentary in tv just now, which pointed out that our education shdn't based on "gred", "score" and how "advance" of our knowledge is. Because new knowledge will always exist to replace our old learnt knowledge. An education should be based on the "moral value" behind to build a good personality. I think this is true, as even we're science student, we must think and learn from our failure, success and hopefully to link our research outcome to benefit the community. This should be the real science. ^^
of coz, since i already commit myself into this job, i shall proceed too whenever there is obstacle. I love my life. hehe, just a little crapping from me again.
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